mmp8 inhibitor  (MedChemExpress)

Journal: Journal of Translational Medicine

Article Title: Micheliolide suppresses epithelial-mesenchymal transition of lens epithelial cells via downregulating matrix metalloproteinase 8 to ameliorate posterior capsular opacification

doi: 10.1186/s12967-026-07890-z

Figure Lengend Snippet: Identification of Micheliolide, a potential MMP8 regulator. A , qRT-PCR analysis of MMP8 expression in capsular tissues 24 hours after ECLE surgery, combined with anterior chamber injection of saline or MCL, n = 3, N = 18. B , Western blot analysis and quantification of MMP8 protein levels in capsular tissues 24 hours after ECLE surgery with anterior chamber injection of saline or MCL, n = 4, N = 20. C , qRT-PCR analysis of MMP8 expression in LECs pretreated with or without 5 μmol/L MCL and stimulated with 10 ng/mL TGF-β2 for 48 hours, n = 6. D , Western blot and corresponding quantification of MMP8 protein expression in LECs pretreated with or without 5 μmol/L MCL followed by stimulation with 10 ng/mL TGF-β2 for 48 hours, n = 4. E , Molecular docking simulation of Micheliolide and MCL, with the interacting MMP8 amino acids represented by blue sticks. F , CETSA was used to assess the thermal instability of the interaction between MMP8 and MCL over the temperature range of 58 °C to 78 °C, n = 4

Article Snippet: To explore the role of Matrix Metalloproteinase 8 (MMP8) in the effect of MCL on EMT, a MMP8 inhibitor (Cat. HY-N1454, MedChemExpress, State of New Jersey, USA), referred to as M8I, was used in the present study.

Techniques: Quantitative RT-PCR, Expressing, Injection, Saline, Western Blot

Journal: Journal of Translational Medicine

Article Title: Micheliolide suppresses epithelial-mesenchymal transition of lens epithelial cells via downregulating matrix metalloproteinase 8 to ameliorate posterior capsular opacification

doi: 10.1186/s12967-026-07890-z

Figure Lengend Snippet: MCL inhibits EMT by regulating MMP8 expression. A , Viability of LECs treated with a gradient concentration of M8I, n = 8. B , Western blot analysis of EMT-related markers in LECs induced by TGF-β2 and treated with MCL alone or in combination with M8I, n = 4. C , Quantitative analysis of the Western blot results shown in ( B ), normalized to GAPDH. D , Coimmunofluorescence staining for αSMA (red) and MMP8 (green) in LECs induced by TGFβ2 and treated with MCL alone or in combination with M8I, n = 3, scale bar = 100um. E , Quantification of immunofluorescence intensity

Article Snippet: To explore the role of Matrix Metalloproteinase 8 (MMP8) in the effect of MCL on EMT, a MMP8 inhibitor (Cat. HY-N1454, MedChemExpress, State of New Jersey, USA), referred to as M8I, was used in the present study.

Techniques: Expressing, Concentration Assay, Western Blot, Staining, Immunofluorescence

Journal: Journal of Translational Medicine

Article Title: Micheliolide suppresses epithelial-mesenchymal transition of lens epithelial cells via downregulating matrix metalloproteinase 8 to ameliorate posterior capsular opacification

doi: 10.1186/s12967-026-07890-z

Figure Lengend Snippet: MCL ameliorates the inflammatory response and suppresses the proliferation, migration, and EMT of LECs following cataract surgery via targeting MMP8. PCS, post-cataract surgery

Article Snippet: To explore the role of Matrix Metalloproteinase 8 (MMP8) in the effect of MCL on EMT, a MMP8 inhibitor (Cat. HY-N1454, MedChemExpress, State of New Jersey, USA), referred to as M8I, was used in the present study.

Techniques: Migration

Journal: Frontiers in Medicine

Article Title: Inhibition of Matrix Metalloproteinase-8 Protects Against Sepsis Serum Mediated Leukocyte Adhesion

doi: 10.3389/fmed.2022.814890

Figure Lengend Snippet: Sequence of primers for Real-Time RT-PCR.

Article Snippet: MMP8 inhibitor (M8I, #sc-311436) was purchased from Santa Cruz (California, USA).

Techniques: Sequencing

Journal: Frontiers in Medicine

Article Title: Inhibition of Matrix Metalloproteinase-8 Protects Against Sepsis Serum Mediated Leukocyte Adhesion

doi: 10.3389/fmed.2022.814890

Figure Lengend Snippet: Identification and GO functional analysis of DEGs and hub genes in GSE64457. (A) The top 100 most significant DEGs are shown in the heatmap (|log2FC| > 1 and adj. p < 0.05). Red indicates a high expression, while green indicates a relatively low expression. (B) The volcano plot shows the 429 DEGs that containing 266 upregulated genes in red (log2FC > 1 and adj. p < 0.05) and 163 downregulated genes in green (log2FC < −1 and adj. p < 0.05). Hub genes are highlighted in the plot. (C) Shows the PPI network of DEGs. Red notes the upregulated genes and green noted the downregulated genes. Triangles represent the hub genes in the network. (D) Hub genes ( MMP8, HP, ARG1, FOLR3, QSOX1, PGLYRP1, OSCAR ) were identified by the overlapping of three algorithms (MNC, MCC, and Degree) in the cytoHubba plugin in Cytoscape and clustered in heatmap (E) . The top 15 GO terms are shown in the dot plots of GO functional analysis of DEGs (F) and hub genes (G) ( p < 0.05). The size of each circle is positive correlated with the counts of the enriched genes in this term. GO, gene ontology; DEGs, differentially expressed genes; PPI, protein-protein interaction; MMP8, matrix metallopeptidase 8; HP, haptoglobin; ARG1, arginase 1; FOLR3, folate receptor gamma; QSOX1, quiescin sulfhydryl oxidase 1; PGLYRP1, peptidoglycan recognition protein 1; OSCAR, osteoclast associated Ig-like receptor; MNC, maximum neighborhood component; MCC, maximal clique centrality.

Article Snippet: MMP8 inhibitor (M8I, #sc-311436) was purchased from Santa Cruz (California, USA).

Techniques: Functional Assay, Expressing

Journal: Frontiers in Medicine

Article Title: Inhibition of Matrix Metalloproteinase-8 Protects Against Sepsis Serum Mediated Leukocyte Adhesion

doi: 10.3389/fmed.2022.814890

Figure Lengend Snippet: LogFC and adj. p Val of 7 hub genes in dataset GSE64457.

Article Snippet: MMP8 inhibitor (M8I, #sc-311436) was purchased from Santa Cruz (California, USA).

Techniques:

Journal: Frontiers in Medicine

Article Title: Inhibition of Matrix Metalloproteinase-8 Protects Against Sepsis Serum Mediated Leukocyte Adhesion

doi: 10.3389/fmed.2022.814890

Figure Lengend Snippet: Survival analysis of hub genes in GSE65682. (A–G) The survival curves of the 7 hub genes in GSE65682. Patients with high expression of FOLR3 and OSCAR had a better prognosis than low expression of these genes. Patients with low expression of MMP8 and ARG1 had a better prognosis than high expression of these genes.

Article Snippet: MMP8 inhibitor (M8I, #sc-311436) was purchased from Santa Cruz (California, USA).

Techniques: Expressing

Journal: Frontiers in Medicine

Article Title: Inhibition of Matrix Metalloproteinase-8 Protects Against Sepsis Serum Mediated Leukocyte Adhesion

doi: 10.3389/fmed.2022.814890

Figure Lengend Snippet: Expression of MMP8 in blood samples from sepsis patients and controls. (A) mRNA expression of MMP8 in neutrophil of patients and controls was measured by q-RTPCR. (B) Protein level of MMP8 in serum of patients and controls was detected by ELISA. * p < 0.05 between control and sepsis group by using t -test.

Article Snippet: MMP8 inhibitor (M8I, #sc-311436) was purchased from Santa Cruz (California, USA).

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay, Control

Journal: Frontiers in Medicine

Article Title: Inhibition of Matrix Metalloproteinase-8 Protects Against Sepsis Serum Mediated Leukocyte Adhesion

doi: 10.3389/fmed.2022.814890

Figure Lengend Snippet: Schematic summary of the in silico and in vitro study of sepsis serum promotes leukocyte adhesion to HUVEC via MMP8.

Article Snippet: MMP8 inhibitor (M8I, #sc-311436) was purchased from Santa Cruz (California, USA).

Techniques: In Silico, In Vitro

Journal: bioRxiv

Article Title: The generation of plasma cells and CD27 − IgD − B cells during Hantavirus infection are associated with distinct pathological findings

doi: 10.1101/723585

Figure Lengend Snippet: (A) Frequency of CD27 − of IgD − B cells or CD23 + B cells 2 h and 4 d after exposure of PBMCs to PUUV (n=6). (B) Quantity of soluble CD27 (sCD27) in patient plasma during acute and convalescent phase (left). Correlation of sCD27 levels with creatinine levels during acute infection (right) (n=23). (C) Total PBMC were incubated with adenosine (Ado), ATP, ATP and suramin (a non-selective antagonist of P2 ATP receptors) (Sur) or ATP and an MMP8 inhibitor (CAS 236403-25-1) (MMp-8i) or medium for 1h. Left panels: representative plots. Upper right panels: decrease in frequency of CD27 + cells in CD20 + IgD − population (left) and compared to medium control experiment (right). Lower right panels: reduced frequency of CD23 + CD20 + B cells (left) and compared to media-only negative control (right) (n=7 donors, average of triplicates shown). (D) Left panel: sCD27 in PBMC culture supernatants in absence or presence of ATP (n=7 donors) Right panel: ratio of sCD27 with or without treatment of PBMC with ATP, right panel (E) quantity of CD27 mRNA in B cells treated with ATP compared to media-only negative control (n=7) (F) Quantity of MMP-8 in patient plasma during acute and convalescent phase (n=23). (G) Longitudinal ATP measurement in fresh plasma (n=8). Black dotted line: average value measured in 22 healthy donors (11 males, 11 females); grey area standard deviation. (H) Creatinine level correlated to ATP represented by quantity of ATP breakdown products during acute infection. Corrected quantity is calculated as ratio acute infection / convalescent phase for each donor (n=23).

Article Snippet: PBMCs from 7 healthy donors were incubated with ATP (Invitrogen, 6.7 mM), adenosine (Sigma, 6.7 mM), ATP and suramin (Sigma, 50 μM), ATP and MMP8 inhibitor (Merck, 10 μM) or control medium for 60 minutes at 37°C 5% CO 2 .

Techniques: Infection, Incubation, Negative Control, Standard Deviation